![](https://parts.igem.org/images/partbypart/icon_primer.png)
Primer
Part:BBa_K4496006:Design
Designed by: Larissa de Souza Kawanisi, Awana da Silva Lima, Paula Macedo Cunha, Gabriel Kimijima Itaziki Group: iGEM22_USP-EEL-Brazil (2022-09-20)
Jararagin gene amplification primers for pPIC9K (FWD)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This primer was designed from the jararhagin sequence optimized for Pichia Pastoris and the plasmid pPICK9k. For this, a 5 amino acid FWD primer tail was designed, using the pPICK9k plasmid map, and joined it with the first 5 amino acids of the jararhagin gene optimized for Pichia pastoris. This sequence was tested for hairpin.
Source
https://parts.igem.org/Part:BBa_K4496000